2014 Help me understand this report
FilmArray® respiratory panel performance in respiratory samples from neonatal care units
Abstract: FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArra…
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Cited by 31 publications
(20 citation statements)
References 19 publications
(23 reference statements)
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“…Respiratory samples were extracted using a QIAsymphony ® instrument with a QIAsymphony ® DSP Virus/Pathogen Midi Kit (Complex 400 protocol) according to the manufacturer's instructions (QIAGEN, Qiagen, Hilden, Germany). Panels of laboratory-developed real-time RT-PCR or real-time PCR [22,23] were used to detect and quantify the following viruses: influenza virus A and B (including subtype determination), human Rhinovirus (hRV), human parainfluenza virus (hPIV) 3, respiratory syncytial virus (RSV) types A and B, human coronavirus (hCoV)-OC43, -229E, -NL63, and -HKU1, human metapneumovirus (hMPV) and human adenoviruses (hAdVs). Extracted samples were also amplified using EV-D68-specific real-time RT-PCR as previously described [24].…”
Section: Ev-d68 Detectionmentioning
confidence: 99%
“…Respiratory samples were extracted using a QIAsymphony ® instrument with a QIAsymphony ® DSP Virus/Pathogen Midi Kit (Complex 400 protocol) according to the manufacturer's instructions (QIAGEN, Qiagen, Hilden, Germany). Panels of laboratory-developed real-time RT-PCR or real-time PCR [22,23] were used to detect and quantify the following viruses: influenza virus A and B (including subtype determination), human Rhinovirus (hRV), human parainfluenza virus (hPIV) 3, respiratory syncytial virus (RSV) types A and B, human coronavirus (hCoV)-OC43, -229E, -NL63, and -HKU1, human metapneumovirus (hMPV) and human adenoviruses (hAdVs). Extracted samples were also amplified using EV-D68-specific real-time RT-PCR as previously described [24].…”
Section: Ev-d68 Detectionmentioning
confidence: 99%
“…Respiratory samples were tested with a panel of laboratory-developed real-time RT-PCR or real-time PCR able to detect and quantify the following viruses [9,10]: influenza virus A and B, including subtype determination, hPIV-3, hRSV types A and B, hCoV types −OC43, −229E, −NL63, and −HKU1, hMPV, HRV and CMV (in a subset of samples).…”
Section: Identification Of Respiratory Virusesmentioning
confidence: 99%
“…In the latter case, follow-up samples were collected at subsequent outpatient clinical evaluations. Respiratory samples were routinely tested using a panel of RT-PCR and real-rime RT-PCR assays [13,19] for human influenza virus types A and B, human metapneumovirus A and B, human respiratory syncytial virus A and B, four human coronaviruses (OC43, 229E, NL63, HKU1), human parainfluenza types 3, human adenovirus, rhinoviruses and enteroviruses.…”
Section: Patients and Samplesmentioning
confidence: 99%
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